Journal: The FASEB Journal

Article Title: The role of chemerin in the regulation of cGAS‐STING pathway in gestational diabetes mellitus placenta

doi: 10.1096/fj.202201611r

Figure Lengend Snippet: FIGURE 1 The differences of placental morphology and mitochondrial structure in GDM patients and normal pregnant women. (A) Representative TEM image of GDM placenta mitochondria (scale bar = 500 nm). (B) Representative TEM image of placenta mitochondria in the control group (scale bar = 500 nm). (C) Specific surface of mitochondria analysis. (D) Representative image of HE staining in GDM placenta (scale bar = 100 μm). (E) Representative image of HE staining in placenta of the control group (scale bar = 100 μm). White arrows indicated placental mitochondria. GDM: gestational diabetes mellitus; TEM: transmission electron microscope; HE: hematoxylin eosin. Data were shown as mean ± SD (n ≥ 3). *p < .05.

Article Snippet: 3.7 | The mitochondrial membrane potential was diminished and phosphorylation of TBK1 and IRF3 protein were enhanced in the HTR- 8/ SVneo cell model To confirm the changes of the mitochondrial membrane potential in the HG and IR cell models, JC- 1 assay kit (C2006, Beyotime Biotechnology, China) was used according to the manufacturer's protocol.

Techniques: Control, Staining, Transmission Assay, Microscopy

Journal: Cell Communication and Signaling : CCS

Article Title: Momordicine-I suppresses head and neck cancer growth by modulating key metabolic pathways

doi: 10.1186/s12964-024-01951-w

Figure Lengend Snippet: M-I treatment inhibits glycolysis and lipogenesis in vivo . MOC2 cells (1 × 10 6 ) were injected subcutaneously into C57BL/6 mice. Tumor‐bearing mice were randomly divided into two groups. The control group received vehicle, and the experimental group received 30 mg/kg/mouse M-I via intraperitoneal injection every day until the end of the experiment. A Tumor volume post-M-I treatment. B Representative images of tumors from control and M-I-treated groups. C Relative mRNA expression of Hk1 , Pdk3 , Fasn and Acly was analysed by qRT-PCR from control or M-I treated tumor RNA.18 s gene was used as internal control. Small bar indicates standard error (*, p < 0.05; **, p < 0.01). n = 3 technical replicates

Article Snippet: To measure the lactate production, glycolysis kit (Dojindo Laboratories-G272) was used according to the instruction.

Techniques: In Vivo, Injection, Control, Expressing, Quantitative RT-PCR

Journal: Nanomaterials

Article Title: Water Filtration Membranes Based on Non-Woven Cellulose Fabrics: Effect of Nanopolysaccharide Coatings on Selective Particle Rejection, Antifouling, and Antibacterial Properties

doi: 10.3390/nano11071752

Figure Lengend Snippet: ( A ) Confocal microscopy micrographs showing BSA adsorption on the surface of the unmodified ( a ), CNC impregnated ( b ), T-CNF impregnated ( c ) and ChNC impregnated ( d ) nonwoven fabrics stained with the Qubit Protein Assay Kit after their incubation with BSA solution for 6 h at pH 7. ( B ) Confocal microscopy micrographs showing E. coli colonization and viability on the surface of the unmodified ( e ), CNC impregnated ( f ), T-CNF impregnated ( g ) and ChNC impregnated ( h ) nonwoven fabrics stained with Live/Dead BacLight Bacterial Viability Kit. Live cells were green stained by SYTO 9 and dead cells were red-stained by PI.

Article Snippet: The proteins were visualized using a Qubit Protein Assay Kit and with a Zeiss LSM 780 Confocal fluorescence microscope (Carl Zeiss MicroImaging GmbH, Oberkochen, Germany).

Techniques: Confocal Microscopy, Adsorption, Staining, Qubit Protein Assay, Incubation

Journal: Cancers

Article Title: Comparison of Cellular Death Pathways after mTHPC-mediated Photodynamic Therapy (PDT) in Five Human Cancer Cell Lines

doi: 10.3390/cancers11050702

Figure Lengend Snippet: Evaluation of mitochondrial membrane potential (Δ ψ m ) after mTHPC-PDT in A-427 ( A ), BHY ( B ), KYSE-70 ( C ), RT-4 ( D ), and SISO ( E ) cells. Cells were treated with solvent (SC) or equitoxic concentrations of mTHPC between 0.07–0.3 µM (equal to the respective IC 90 in ), illuminated with 1.8 J/cm 2 or left in the dark, and stained with the cationic dye JC-1 after an incubation period of 6 h post illumination or incubation in the dark. JC-1 monomers and aggregates were visualized with a fluorescence microscope equipped with a 63× oil/1.4 NA objective. JC-1 aggregates within active mitochondria are shown in red, whereas cytosolic JC-1 monomers display a green fluorescence. A decrease in red fluorescence indicates a decline in Δ ψ m , which is a sign of early apoptosis. Fluorescence images were captured with the FITC filter cube (green; λ Ex/Em = 460–500/512–542 nm) and the RHOD filter cube (red; λ Ex/Em = 541–551/565–605 nm). Solvent-treated and non-illuminated cells served as the negative control with active mitochondria. As a positive control, cells were treated with 50 µM CCCP, a mitochondrial oxidative phosphorylation uncoupling agent.

Article Snippet: Evaluation of Mitochondrial Membrane Potential (Δ ψ M ) Mitochondrial membrane potential (Δ ψ m ) was detected with the BD MitoScreen JC-1 kit (BD Biosciences, San Diego, CA, USA) according to the kit instructions (modified for fluorescence microscopy).

Techniques: Staining, Incubation, Fluorescence, Microscopy, Negative Control, Positive Control